Occurrence of extended-spectrum betalactamase (ESBL) and carbapenemases among ampicillin-resistant enterobacteriales recovered from a municipal raw sewage in Minas Gerais, Brazil

Introduction: The capacity of resistance to β-lactam among enterobacteriales is notable, mainly into water environment. Herein, many species this family have the ability carrier and produce β-lactamases enzymes, such as extended-spectrum (ESBLs) carbapenemases. However, contrary clinical settings, where distribution resistant bacteria well documented, evidence resis-tant pathogens in domestic sewage has been little explored, especially Brazil. Thus, we aimed investigate occurrence ESBL carbapenemases between ampicillin-resistant recovered from a municipal raw Minas Gerais, Methods: Enterobacteriales were isolated samples on MacConkey agar supplemented with ampicillin. Species identifica-tion was performed by biochemical morphological methods profile determined Kirby-Bauer test. production carbapen-emase investigated all isolates phenotypic tests. Results discussion:A total 45 ampicillin (37 Escherichia coli, four Klebsiella pneumoniae, one oxytoca, Citrobacter freundii Pantoea agglomerans). Most showed high suscep-tibility (14/45, 31.1 %), however E. coli decreased susceptibility imipenem detected (2/37; 2.7 %). ESBL-positive mostly identified (10/45; 22.2 but no positive carbapenemase. Conclu-sion: Domestic an important source β-lactams determinants